P1BS.16 - Impedimetric Cortisol Biosensor with in-Situ Reduced Hexaammineruthenium as Redox-Probe
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-1 - Biosensors
- Author(s)
- J. Schrattenecker, R. Heer, E. Melnik, R. Hainberger - AIT Austrian Institute of Technology GmbH, Vienna (Austria), G. Fafilek - Institute of Chemical Technologies and Analytics, Technische Universität Wien, Vienna (Austria)
- Pages
- 505 - 506
- DOI
- 10.5162/IMCS2018/P1BS.16
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Cortisol is an important biomarker to monitor the stress level. In this work, we present a cortisol biosensor using electrochemical impedance spectroscopy as read-out method. It is known that hexacyanoferrat(III/II) can corrode gold electrodes. To overcome this problem, we chose a different path. By applying a DC-bias to the EIS measurement, the redox-pair [Ru(NH3)6]3+/2+ was generated insitu by reduction of the hexaammineruthenium(III) on the working electrode. A sensor consisting of a three electrode system (working, counter, reference electrode) was used to ensure the correct application of the bias voltage. The realized impedimetric sensor had a linear range from 1*10-11 to 1*10-7 mol/L and a linear correlation coefficient of 0.972.