P1BS.18 - Electrochemical Sensing and Consumption of Intracellular NADH in Mammalian Cells for their Applications
- Event
- 17th International Meeting on Chemical Sensors - IMCS 2018
2018-07-15 - 2018-07-19
Vienna, Austria - Chapter
- P-1 - Biosensors
- Author(s)
- H. Shinohara, M. Suga - Graduate School of Science and Engineering for Research, University of Toyama, Toyama (Japan), H. Taketsu, M. Kyuji - Graduate School of Science and Engineering for Education, University of Toyama, Toyama (Japan)
- Pages
- 509 - 509
- DOI
- 10.5162/IMCS2018/P1BS.18
- ISBN
- 978-3-9816876-9-9
- Price
- free
Abstract
Electrochemical sensing of intracelluar NADH was performed with 1-Methoxy-PMS as a cell membrane-permeable oxidizing mediator to monitor viability and number of mammalian cells. The electrochemical sensing for intracellular NADH was applied to evaluate the inhibition effect of oxamate to intracelluar lactate dehydrogenase (LDH). Futhermore, electrochemical consumpsion of intracelluar NADH to induce cell death also examined on the cell-adhered trasparent electrode by constant oxidizing voltage application with 1-Methoxy-PMS as a redox cycling mediator.