A5.3 Single Cell Immobilization at High Flow Rates Using 2PP-Traps in a Microfluidic Channel
- SMSI 2020
(did not take place because of Covid-19 virus pandemic)
- SMSI 2020 - Sensors and Instrumentation
- A5 Sensor Materials and Technologies
- S. Reede, M. Vellekoop - University of Bremen, Bremen (Germany), H. Müller-Landau, N. Matscheko, U. Rant - Dynamic Biosensors, Martinsried/ Planegg (Germany), F. Lucklum - Technical University of Denmark, Kgs. Lyngby (Denmark)
- 81 - 82
This paper presents robust single cell traps fabricated in a microfluidic channel by two-photon polymerization (2PP) that withstand a very high average flow velocity of 1 m/s. The traps consist of a direct laser written thin grid structure allowing a good flow around the captured cells as well as optical measurements. Therefore, they enable the characterization of binding kinetics between active sub-stances and cells. The traps have the shape of half a circular cylinder and capture the cells hydrody-namically. Jurkat cells with a diameter of 15 μm are captured in every trap within the first seconds of sample injection.